We further confirmed the key role of the cGAS enzymatic activity for restraining FMDV infection using recombinant pocGAS-eGFP proteins that were overexpressed either in the wild-type version or as the E200A/D202A inactive form in swine IBRS2 and WSL cells that were subsequently infected with FMDV at different MOI (Supplementary Fig. 4a, b). This evidence concerns the gene CGAS and infection.