Accordingly, we observed that the glyCAFs lose their capability to block T-cell migration both in the presence of GLUT1i or when the expression of GLUT1 is silenced by a short hairpin RNA (shRNA) targeting Slc2a1 (Glut1-KD) (Fig. 5d and Supplementary Fig. 5f), reinforcing the idea that glyCAF and their glycolytic properties are the main determinants for the trafficking of T cells in the tumor mass. The gene discussed is SLC2A1; the disease is neoplasm.