Consistent with other ALS and ALS/FTD mouse models lacking mutant protein overexpression40, overt phenotypes related to motor neuron dysfunction (Supplementary Fig. 12c, d) and gliosis were not observed in PFN1 C71G+/− mice aged to ~600 days (Supplementary Fig. 13). Here, PFN1 is linked to amyotrophic lateral sclerosis.