Our data show that, in AD brain, HSPB1 is predominantly expressed in astrocytes, in line with previous immunohistochemistry studies in AD and other tauopathies, where HSPB1 expression was mostly attributed to glial cells (24,63), and with recent single-cell transcriptomic studies in human AD brain (64–66). Here, HSPB1 is linked to Alzheimer disease.