Additionally, HuR SUMOylation was studied in the context of hypoxia, a hallmark of many solid tumors showing an aggressive phenotype,81 in addition to a well-known inductor of protein SUMOylation.82 In this case, the HuH-7 human hepatoma cell line was transiently transfected with the constructs expressing V5-tagged WT or K120/182R HuR and incubated under hypoxic conditions (1% O2) for 24 h. The gene discussed is ELAVL1; the disease is hepatocellular carcinoma.