To evaluate the effect of CCL1 on cell motility of CRC, the scratch test conducted on Colo320DM cells transfected with Ns siRNA as a negative control showed significant cell motility upon treatment with rCCL1 (10 ng/mL), whereas CCR8 knockdown groups transfected with siRNA showed low cell motility upon treatment with rCCL1 (Figure 6A,B). The gene discussed is CCL1; the disease is colorectal carcinoma.