To investigate whether the CCL1‐CCR8 axis is involved in tumour invasion and metastasis through epithelial‐mesenchymal transition (EMT) and tumour angiogenesis in Colo320DM cells, RT‐qPCR with siRNA treatment was conducted to evaluate the expression of CDH1, MMP‐2, MMP‐9 and VEGFA mRNA. This evidence concerns the gene MMP9 and neoplasm.