To confirm the difference in the characteristics of TAMs in CRC and CRLM, double immunofluorescence staining of CCL1 and CD68, as pan macrophage markers, showed CD68 as positive in macrophages and stroma of CRC and CRLM (Figure 2C [a,b], Figure 2D [a]), and CCL1 positive cells were almost confined to CD68 positive macrophages in CRC compared with those in CRLM (Figure 2C [c,d], Figure 2D [b]). The gene discussed is CCL1; the disease is colorectal carcinoma.