Cell clusters were annotated based on the average expression of canonical marker genes, identifying melanoma cells, immune cells (such as myeloid cells, plasmacytoid dendritic cells (pDCs), CD8+ T cells, CD4+ T cells, regulatory T cells (Tregs), natural killer/natural killer T (NK/NKT) cells (Figure S2), and B cells), fibroblasts, endothelial cells, and epithelial cells (Figure 1C-D). The gene discussed is CD4; the disease is melanoma.