ACTA2 and cancer: Through unsupervised clustering and classical markers, we identified six subgroups of interstitial cells (Figure 5A): vascular endothelial cells with elevated Von Willebrand factor (VWF) expression, lympho-endothelial cells with heightened prospero homeobox 1 (PROX1) expression, myofibroblastic cancer-associated fibroblast (myoCAFs) primarily expressing actin alpha 2 (ACTA2) and myosin IB (MYO1B), and iCAF characterized by chemokines and immunosuppressive factors, each exhibiting distinct gene expression patterns (Figure 5B-C).