We analyzed the effect of CCl4-induced liver fibrosis and splenectomy on the percentage and number of hepatic classical (CD45+ CD11b+ Ly6G- CX3CR1low Ly6Chigh) Mon/Mφs and non-classical (CD45+ CD11b+ Ly6G- CX3CR1high Ly6Clow) Mon/Mφs by flow cytometry. The gene discussed is ITGAM; the disease is Hepatic fibrosis.