The activation of the TLR/NF-κB signaling pathway leads to osteoclast differentiation and maturation, which in turn accelerates bone destruction, while inhibition of the TLR4/NF-κB pathway could inhibit the differentiation and maturation of osteoclasts and regulate osteoblast differentiation, thus attenuating the occurrence of osteoporosis. This evidence concerns the gene TLR4 and osteoporosis.