Subsequent western blotting assay also revealed no visible alteration by overexpressing TMEM120B-FL and TMEM120B-NLS-mutant in SK-BR-3 cells, respectively (Additional file 3: Fig. S8F).Third, TMEM120B promoted breast cancer cells by binding with MYH9 which was previously reported as a cytosolic protein [33, 34].Therefore, from our current results, cytosolic TMEM120B may serve as an oncogenic protein rather than nuclear one, however, the function of TMEM120B should be further investigated in the future. The gene discussed is TMEM120B; the disease is breast cancer.