Finally, we overexpressed TMEM120B, TMEM120B-∆CCD, MYH9, MYH9-∆CCD alone or co-transfected TMEM120B + MYH9, TMEM120B-∆CCD + MYH9, and TMEM120B + MYH9-∆CCD into breast cancer cells. Here, TMEM120B is linked to breast cancer.