Hippo and PI3K-AKT signaling were chosen for further studies as they are both proven to be crucial in maintaining stemness of breast cancer cells [48–50].Subsequent western blotting indicated that levels of phosphorylated AKT, phosphorylated mTOR, and TAZ were significantly increased or decreased, and YAP level was slightly increased or decreased after the overexpression or depletion of TMEM120B in SK-BR-3 or MDA-231 cells, respectively (Fig. 4C). Here, AKT1 is linked to breast cancer.