Flow cytometry also indicated that the ratio of ALDH1-positive cells elevated more than the other groups upon co-transfecting TMEM120B + MYH9 (Additional file 3: Fig. S6F).Our results indicated that the TMEM120B–MYH9 interaction may enhance breast cancer stemness by activating the β1-integrin-FAK-TAZ-mTOR signaling axis. Here, PTK2 is linked to breast cancer.