Overexpressing of TMEM120B promoted breast cancer 3D invasion, which all indicated that TMEM120B were involved in modulating mechanical force transduction.β1-integrin cycling and FA assembly were enhanced in the TMEM120B–MYH9 interaction, thus promoted translocation of TAZ into nucleus as well as subsequent elevation of phosohorylation of mTOR. The gene discussed is MYH9; the disease is breast cancer.