Additionally, xenograft assays revealed that tumor volumes significantly increased in SK-BR-3 cells overexpressing TMEM120B (Fig. 2F), whereas the number of lung metastases visibly increased in the ectopic TMEM120B group, however there were no obvious changes in metastasis of liver, brain, kidney and heart (Fig. 2G, Additional file 3: Fig. S2G). This evidence concerns the gene TMEM120B and neoplasm.