TP53 and cancer: Next, we assessed the affected genes and the functional impact of the called variants (see Table 2 and Additional file 1: File S1) and translated our information from DNA to protein level, e.g., we identified TP53 mutations such as p.R273*, the most frequently mutated TP53 codon in human cancers, and p.R175H, the most frequent TP53 mutation in human cancers.