Our study concluded that high expression of miR-26a-5p targeting RFC1 and miR-636 targeting RFC5 expression in the mismatch repair, DNA replication, nucleotide excision repair pathway is expected to be a potential biomarker for detecting CRC at an early stage and small molecule CHEMBL430483 can be developed for the treatment of the disease. Here, RFC5 is linked to colorectal carcinoma.