In order to determine whether NEK2 in gastric cancer cells regulates HMOX1 expression through Keap1/Nrf2 signaling and then affect the ferroptosis sensitivity, firstly, we analyzed the effect of NEK2 on the degradation of HMOX1 protein, and the results showed that NEK2 knockdown did not affect the degradation level of HMOX1 protein (Fig. 4A), suggesting that NEK2 might regulate the synthesis process of HMOX1. The gene discussed is HMOX1; the disease is gastric cancer.