In this study, we found that inhibiting NEK2 could increase the levels of Fe2+, ROS and lipid peroxidation, and these phenomena were recovered after HMOX1 knockdown, confirming that inhibiting NEK2 and then increasing HMOX1 promote the process of ferroptosis in gastric cancer, which is related to excessive Fe2+ accumulation. This evidence concerns the gene NEK2 and gastric cancer.