To investigate the impact of SCGB1D2 on Bb establishing infection in vivo, we injected C57BL/6 J female mice aged at 10 weeks at the time of infection intradermally with Bb incubated with either SCGB1D2 or SCGB3A1, a secretoglobin that does not associate with Lyme disease. Here, SCGB3A1 is linked to Lyme disease.