Thus, in line with our in vivo data in scrub typhus patients, these experiments show a marked upregulation of DLL1 and a reciprocal downregulation of NOTCH4. Moreover, they show prominent downstream readout of Notch signaling such as CREBBP and HEY1, both identified as a specific molecular target for Notch signaling. This evidence concerns the gene DLL1 and scrub typhus.