When rapamycin was present, HEK293T cells electroporated with the highest amounts of Siglec6 mRNA stimulated release of 40–60 ng/mL IFN-γ from DARIC-VHH1 T cells, which corresponds to approximately 10% of the amount released following coculture with CD33+ AML cells. This evidence concerns the gene CD33 and acute myeloid leukemia.