To understand the effect of chronic hypoxia on glutaminolysis in HSCs, the expression of two key enzymes, GLS1 (which decomposes glutamine) and SlC38A1 (which facilitates glutamine transfer) in the C-NASH and M-NASH groups was quantified by qRT-PCR (Figures 2K, L). Here, SLC38A1 is linked to metabolic dysfunction-associated steatohepatitis.