Representative confocal images with microglia and TREM2 co-localization and high-resolution 3D reconstructions of the confocal z-stacks and orthogonal images to show Iba1 and TREM2 co-localization in Tg-Saline and Tg-TfRMAb-TNFR 3xTg-AD mice are shown in Fig. 5A. Quantification of the Iba1-positive area showed no change between WT-Saline and Tg-Saline mice or between Tg-Saline mice and Tg-TfRMAb-TNFR mice in the plaque-free or plaque-bearing regions of the hippocampus (Fig. 5B). This evidence concerns the gene AIF1 and Alzheimer disease.