RIC8B and infection: We used a TetON-based system that consisted of two components: for tight control of Tet-dependent transgene expression in neurons, one viral construct contained the human synapsin (hSyn) promoter controlling the expression of the reverse tetracycline-controlled transactivator (rtTA) together with the Tet repressor (TetR) and the fluorescent protein Kusabira Orange (KO) that serves as an infection marker [46, 47].