This work identified two drug targets for hyperparathyroidism: PIK3C3 and SLC40A1 employing a variety of MR methods (MR-Egger, Wald ratio/IVW, weighted median method, Cochran’s Q heterogeneity test, horizontal multiplicity test, bidirectional MR analysis, Steiger test, colocalization analysis and multivariable MR analysis). This evidence concerns the gene SLC40A1 and hyperparathyroidism.