By this finding, the regulators involved in mTOR signaling (RHEB and LAMTOR4) and S‐phase (RFC2, PSMC2, and ORC5) were more frequently gained and highly expressed in TLS‐low group, indicating that mTOR signaling activation and cell cycle progression in tumor cells might result in reduced TLS abundance in HCC tissues and unfavorable prognosis in HCC‐LT. Here, RHEB is linked to hepatocellular carcinoma.