In the ninth week, a cocktail of genome targeting reagents, including pCas9D10A-EGFP and pCTNNB1-mCherry-tk-gRNA, mixed with in vivo-JetPEI delivery reagents were injected into the tail vein of the animals 3 times a week for HSV1-tk insertion into the cancer genome. This evidence concerns the gene TKT and cancer.