TKT and neoplasm: Similar effects were seen with the constructs of pCas9D10A-EGFP and pSLAM-HSV1-tk-mCherry-gRNA on HUH7-xenografted SCID mice: The treatment reduced the tumor burden by an average of 17.6-fold (p < 0.01, Figure 4G) and decreased incidence of metastasis/invasion by 4-fold (p < 0.05, Figure 4H).