PDCD1 and neoplasm: After batch correction, both visual and quantitative evaluations in cell identity and transcriptional signatures showed very similar population clustering to recently published datasets of adoptively transferred tumor specific T cells found in the TdLN (Figure S4A).22 To confirm that the TdLN-derived CD8+ PD-1+ T cells in our dataset were clonally related to the CD8+ T cells within the tumor, we performed bulk-TCR sequencing of sorted CD8+ PD-1+ T cells from paired TdLN and tumor mice samples.