To further identify cytokines affecting non/low response to hepatitis B immunization, we selected 14 cytokines that were significantly differentially expressed between the two groups at birth for LASSO regression analysis and construction of a ROC clinical prediction model, which identified 4 critical cytokines, respectively, IL-5, HGF, IL-12p40 and β-NGF. This evidence concerns the gene IL5 and hepatitis B virus infection.