COL4A1 and ITGA3 genes were selected from list of GSEA Figures 3A, B to validate the RNA sequencing analysis based on their role in proliferation, EMT and stemness of breast cancer cells (Halsted et al., 2008; Jin et al., 2017; Wang et al., 2020b; Zhang et al., 2020). This evidence concerns the gene COL4A1 and breast cancer.