In this study, we found that MIR99AHG could emerge as a tumor regulator to play a role in malignant progression via hsa-miR-21-5p-EHD1 axis in LC, while it also had direct base-pairing interaction with EHD1. The lncRNA that we identified, MIR99AHG, was identified as a long non-coding RNA located on chromosome 21q21.1. This evidence concerns the gene MIR99AHG and laryngotracheoesophageal cleft.