Furthermore, immunohistochemical staining assays showed that Ki67 expression was significantly decreased in tumor tissue of LAS treated tumor bearing nude mice compared to that of negative controls, while TUNEL positive cells markedly increased, suggesting that LAS can significantly inhibit the BC cells proliferation and induce the apoptosis of breast cancer cells in vivo (Fig.6A-C). This evidence concerns the gene MKI67 and breast carcinoma.