To provide a theoretical foundation for the treatment of NSCLC patients with X-ray and carbon ion combined with ICI, we conducted an in-vitro experiment to investigate the radio-immune effects and its mechanism of different LET rays combined PD-1 inhibitors (murine PD-1) to irradiated and abscopal tumors on lung adenocarcinoma. The gene discussed is PDCD1; the disease is non-small cell lung carcinoma.