Our findings arein agreement with several previous reports, inwhich the actions of the BPCs SAD-128 or MB327 were investigated bysingle-channel patch clamp analysis of nAChRs in frog and murine musclecells6,13 as well as in CN21 cells,10 a human rhabdomyosarcoma cell line stably transfected withthe adult ε-nAChR subunit.41 In contrastto another electrophysiological study20 that found a positive allosteric modulation of CHO cell-expressedhuman α7 nAChRs by BPCs, we did not see PAM-like effects. Here, CHRNA7 is linked to rhabdomyosarcoma.