Furthermore, to analyze the biological roles of PHLDA1 in neuroblastoma, we performed quantitative proteomic analysis of PHLDA1-silenced vs. control IMR-32 cells and found that PHLDA1 silencing causes the most spectacular increase in biological functions of mitochondria (Figure 4C), whereas proteins associated with cellular components of the cytoplasm are decreased in the cells (Figure 4D). This evidence concerns the gene PHLDA1 and neuroblastoma.