Through cell-cell interaction analysis, we observed that outgoing and incoming signals of the NEGR pathway were highly enriched in obesity relevant cell subpopulations such as Lhx1, Nr5a1 and Sst in offspring from maternal HFD groups which further highlighted the higher chance of developing obesity and related metabolic disorders in offspring raised by mothers fed HFD. The gene discussed is LHX1; the disease is Other metabolic disease.