PLOD1 and Anxiety: In this study, we used behavioral, patch-clamp electrophysiology, chemogenetic, and optogenetic approaches to clarify the response of the LH and mPFC to stress, confirmed the structural connection between the LH and mPFC, and investigated the role of the LHOrx–mPFC pathway in chronic stress-induced anxiodepression symptoms, including anxiety, anhedonia, and despair behaviors.