The results of RT-qPCR and Western blot demonstrated an enhancement in the mRNA and protein expression of SMAD3, ITGA6, PI3K protein expression and Akt phosphorylation level yet unchanged total Akt protein expression in the tumor tissues of mice treated with H460/oe-SMAD3 + Gy or H460/CAFs + Gy. The gene discussed is SMAD3; the disease is neoplasm.