An MT inhibitor (4-P-PDOT) and a DHT inhibitor (finasteride) were used to establish a model with dual deficiency of MT and DHT in normal mice for 21 days (Fig. 6A), which revealed the effects of MT and DHT on sleep-deprived mice, further verifying the potential mechanism of MT and DHT deficiency in the induction of prostatitis. The gene discussed is MCAT; the disease is medical procedure.