The Tet-On inducible system was quantified using serial Dox concentrations from 0.1 to 500 ng/mL and the results demonstrated that these cells displayed a range of CAIX expression, covering MMNK-1 healthy cells (1,824 CAIX molecules per cell) to skrc-59 tumor cells (123,789 CAIX molecules per cell) and providing an isogenic cell line that can be used for cytotoxicity assessment (Fig. 2B and S1B). This evidence concerns the gene CA9 and neoplasm.