To determine the biological function of POU6F2-AS1 in CRC cells, we constructed two siRNAs (si-POU6F2-AS1#1 and si-POU6F2-AS1#2) and one shRNA (sh-POU6F2-AS1) targeting POU6F2-AS1 and one overexpression plasmid (POU6F2-AS1). The gene discussed is PTGDR; the disease is colorectal carcinoma.