POU6F2 and colorectal carcinoma: To understand the underlying biological pathways by which POU6F2-AS1 facilitates CRC tumorigenesis and growth, we performed RNA-seq on CRC cells transfected with the POU6F2-AS1 overexpression plasmid or empty vector and found a total of 2030 differentially expressed genes (DEGs) (P < 0.05, |log FC| ≥ 1) were detected (Fig. 3A).