To further elucidate the binding regions between POU6F2-AS1 and YBX1, we constructed one biotinylated full-length POU6F2-AS1 construct and four biotinylated fragments of POU6F2-AS1 (F1: full-length sense of POU6F2-AS1; F2: full-length antisense of POU6F2-AS1; F3: Exon1 + 2, 1–116 nt; F4: Exon2 + 3, 14–195 nt; F5: Exon4, 196–381 nt) for RNA pull-down experiments with CRC cell lysates (Fig. 4H). Here, YBX1 is linked to colorectal carcinoma.