Moreover, fluorescence in situ hybridization (FISH) assays of a tissue microarray (TMA) of 60 pairs of CRC tissues and ANTs stained with the POU6F2-AS1 probe also showed that the expression of POU6F2-AS1 was significantly upregulated in CRC tissues (Fig. 1H, Fig. S1F, G). This evidence concerns the gene POU6F2 and colorectal carcinoma.