TLR4 and myocardial infarction: In principle, our findings are well compatible with results from a similar porcine MI model [74], where tissue gene expression analysis by qPCR revealed early activation of the pro-inflammatory TLR-4 pathway of the innate immune response, involving upregulation of key downstream mediators, such as TNF, IL6, and ultimately NFKB1 within hours of reperfusion.