VPS37A and osteosarcoma: To assess the role of hydrophobic membrane-binding motifs (HMs), namely 3WLFP and 137FPYL, in targeting the ESCRT-I complex to the phagophore, the VPS37A wildtype and a mutant with both the 3WLFP and 137FPYL motifs mutated to DDDD (HMsDM) were transduced into VPS37A knockout (KO) U-2 OS human osteosarcoma cells6.