GPC3 and hepatocellular carcinoma: Furthermore, the expression levels of the HCC clinical molecular markers Gpc3 and Afp, the proliferation marker Mki67, and the fibrosis marker Col1a1 were determined by RT-qPCR (Fig. 1D); the results showed a significantly increased expression of all genes determined in the HCC group when compared with the control group; moreover, the administration of IFC-305 reversed this effect.