To explore the role of ATF3-CBS signaling axis in ferroptosis, we knocked down ATF3 in CRC cells and found that CBS expression is reduced in ATF3 KD cells (Fig. 4H and Fig. S5A), resulting in reduced cell proliferation (Figs. S5B and C) and increased ferroptosis (Fig. 4I and J, Figs. S5D–I). This evidence concerns the gene CBS and colorectal carcinoma.