Also, the significant reduction that occurred with MFS either alone or combined with NLCs did not concede with Palić et al. [49, 50], who stated that MFS in the treatment of Leishmania targeted the Th-1 signaling, increasing the concentrations of pro-inflammatory Th-1 cytokines, such as IFN-γ and IL-12 and induced a shift of the macrophage phenotype, which is essential for the clearance of intracellular pathogens which we refer to the use of different infection models. Here, IFNG is linked to infection.