OME pretreatment relieved GSH-Px, GSH, MPO, and TNF-α contents compared with the EtOH group (P < 0.05), while MDA content was significantly increased (P < 0.05) and GSH-Px and IL-1β contents were clearly reduced (P < 0.05) compared with the PBS group. The gene discussed is TNF; the disease is otitis media with effusion.