AKT1 and gastric cancer: Western blotting analysis showed that siRNA-mediated silencing of CRISPLD1 significantly decreased the levels of p-PI3K and p-AKT in HGC-27 and BGC-823 cells, indicating that knockdown of CRISPLD1 can inhibit the activation of the PI3K-AKT signaling pathway in GC cells (Fig. 5E and F).