ANXA5 and acute myeloid leukemia: Therefore, to mimic the in vivo situation and verify the impact of the miR-103a-2-5p on CD8 + T cells, we co-cultured AML cells transfected with miR-103a-2-5p mimics or miR-NC mimics and HLA-matched PBMCs (Additional file 5: Table S3) for 24 h or 72 h and then examined apoptotic CD8 + T cells by staining the sample with Annexin V-APC/PI-PE.