PDCD1 and acute myeloid leukemia: To further determine the potential mechanism of miR-103a-2-5p on CD8 + T cells, we also performed flow cytometry to analyze immune checkpoint receptors including PD-1, CTLA-4, LAG-3, and TIM-3 on T cells in the miR-103a-2-5p/NC mimics AML co-culture system.