Moreover, various cancer-related pathways and terms, such as “MAPK signaling,” “PI3K-Akt signaling pathway,” and “Rap1 signaling pathway”, “Pathways in cancer,” “Prostate cancer,” “Renal cell carcinoma,” “Lung cancer,” and “Breast Cancer,” were also identified, along with immune-related pathways like “T cell and B cell receptor pathway” and “Helper T cell differentiation.” Furthermore, the presence of “PD-L1 expression” and the “PD-1 checkpoint pathway in cancer” indicate that the genes targeted by miRNAs are directly associated with immunotherapy. The gene discussed is CD274; the disease is breast cancer.