The ROC analysis results showed that AQP9 (Fig. 7B), ETS2 (Fig. 7C), PLAU (Fig. 7H), PLEK (Fig. 7I), SOCS3 (Fig. 7J), and TNFRSF1B (Fig. 7K) genes can distinguish between PCOS and control samples with AUC values greater than 0.9. This evidence concerns the gene PLEK and polycystic ovary syndrome.