In addition, the expression of miR-5100 correlated positively with HIF1α level in HNSCC tissues according to the results of IHC and ISH assays (Fig. 1I), and the analysis of Kaplan-Meier Plotter database showed that higher miR-5100 expression predicted shorter overall survival of HNSCC patients (Fig. 1J). This evidence concerns the gene HIF1A and head and neck squamous cell carcinoma.