We furtherly double-stained macrophages (CD163+ CD68+), helper T cell (CD3+ CD4+), cytotoxic T cell (CD3+ CD8+), and Treg cell (CD4+ FOXP3+) of subcutaneous tumor tissues and examined the cells using immunofluorescence (IF) (Fig. 5; Fig. S3). This evidence concerns the gene CD68 and neoplasm.