TMPRSS2 and neoplasm: Next, we used inferCNV (Supplementary Figure 1) to explore the single-cell RNA-seq data from tumor, distinguished tumor epithelial cells, and made further sub-clustering, resulting in five cell subgroups and marking their cell numbers: C0 TMPRSS2+ Tumor EPCs(1266), C1 ANKRD36C+ Tumor EPCs(919), C2 HK2+ Tumor EPCs(489), C3 PLP2+ Tumor EPCs(440), C4 MKI67+ Tumor EPCs(39) (Figure 2A, upper left), and showed the relationship between five cell subgroups and cell cycle stages (Figure 2A, upper right), HPV infection (Figure 2A, lower left) and sample source (Figure 2A, lower right).