Fluorophore-conjugated rVAR2, CD45 and CD66b was used for microscopic detection of the captured cells. CTCs classified as rVAR2+/CD45-/CD66b-Targeted whole exome sequencing identified gene with cancer indicative mutations (RB1, TP53/EPM2AIP1, and TP53/ALK). Here, EPM2AIP1 is linked to cancer.